Lin Guo was a Post-Doctoral Fellow and Research Associate in the lab from August 2012 to February 2019. She received her bachelor’s degree in Chemistry from Peking University in 2005 and her Ph.D. in Chemistry in 2011 from The University of Pennsylvania where she developed spectroscopic methods to study protein folding and peptide-membrane interaction. In 2014, Lin won a post-doctoral fellowship from the Ellison Medical Foundation and American Federation for Aging Research. In 2016. Lin won an Alzheimer's Association Research Fellowship. In 2017, Lin won a Target ALS Springboard Fellowship. In 2018, Lin was selected as a Stat Wunderkind. In 2019, Lin established her own research program as an Assistant Professor in the Department of Biochemistry & Molecular Biology at Jefferson University.
Guo, L.*^, J.R. Mann*, J.C. Mauna°, K.E. Copley°, H. Wang°, J.D. Rubien°, H.M. Odeh, J. Lin, B.L. Lee, L. Ganser, E. Robinson, K.M. Kim, A.C. Murthy, T. Paul, B. Portz, A.M. Gleixner, Z. Diaz, J.L. Carey, A. Smirnov, G. Padilla, E. Lavorando, C. Espy, Y. Shang, E.J. Huang, A. Chesi, N.L. Fawzi, S. Myong, C.J. Donnelly^, and J. Shorter^. (2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. bioRxiv. doi:10.1101/2023.09.04.555754. pdf file link (*Co-first author. °Co-second author. ^Co-corresponding author).
Sharma, K., S. Banerjee, D. Savran, C. Rajes, S. Wiese, A. Girdhar, N. Schwierz, C. Lee, J. Shorter, M. Schmidt, L. Guo, and M. Fändrich. (2023). Cryo-EM structure of the full-length hnRNPA1 amyloid fibril. J. Mol. Biol. 435(18):168211. pdf file link
Kim, H.J.*, P. Mohassel*, S. Donkervoort, L. Guo, K. O’Donovan, M. Coughlin, X. Lornage, N. Foulds, S.R. Hammans, A.R. Foley, C.M. Fare, A.F. Ford, M. Ogasawara, A. Sato, A. Iida, P. Munot, G. Ambegaonkar, R. Phadke, D.G. O’Donovan, R. Buchert, M. Grimmel, A. Töpf, I.T. Zaharieva, L. Brady, Y. Hu, T.E. Lloyd, A. Klein, M. Steinlin, A. Kuster, S. Mercier, P. Marcorelles, Y. Péréon, E. Fleurence, A. Manzur, S. Ennis, R. Upstill-Goddard, L. Bello, C. Bertolin, E. Pegoraro, L. Salviati, C.E. French, A. Shatillo, F.L. Raymond, T. Haack, S. Quijano-Roy, J. Böhm, I. Nelson, T. Stojkovic, T. Evangelista, V. Straub, N.B. Romero, J. Laporte, F. Muntoni, I. Nishino, M.A. Tarnopolsky, J. Shorter, C.G. Bönnemann, and J.P. Taylor. (2022). Heterozygous frameshift variants in hnRNPA2B1 cause early-onset oculopharyngeal muscular dystrophy. Nat. Comm. 13(1):2306. pdf file link (*Co-first author).
Gottlieb, L., L. Guo, J. Shorter, and R. Marmorstein. (2021). N-alpha-acetylation of Huntingtin protein increases its propensity to aggregate. J. Biol. Chem. 297(6):101363. pdf file link
Beijer, D.*, H.J. Kim*, L. Guo*, K. O’Donovan, I. Mademan, T. Deconinck, K Van Schil, C.M. Fare, L.E. Drake, A.F. Ford, A. Kochański, D. Kabzińska, N. Dubuisson, P. Van den Bergh, N.C. Voermans, R.J.L.F. Lemmers, S.M. van der Maarel, D. Bonner, J.B. Sampson, M.T. Wheeler, A. Mehrabyan, S. Palmer, P. De Jonghe, J. Shorter, J.P. Taylor, and J. Baets. (2021). Characterization of HNRNPA1 mutations defines diversity in pathogenic mechanisms and clinical presentation. JCI Insight. 6(14):e148363. pdf file link (*Co-first author.).
Robinson, E., J. Shorter, and L. Guo^. (2020). Karyopherin-β2 inhibits and reverses aggregation and liquid-liquid phase separation of the ALS/FTD-associated protein FUS. Bio Protoc. 10(16):e3725. pdf file link (^Corresponding author)
A.G. Niaki*, J. Sarkar*, X. Cai, K. Rhine, V. Vidaurre, B. Guy, M. Hurst, J.C. Lee, H.R. Koh, L. Guo, C.M. Fare, J. Shorter, and S. Myong. (2020). Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations. Mol. Cell. 77(1):82-94. pdf file link (*Co-first author).
F. Gasset-Rosa, S. Lu, H. Yu, C. Chen, Z. Melamed, L. Guo, J. Shorter, S. Da Cruz, and D.W. Cleveland. Cytoplasmic TDP-43 de-mixing independent of stress granules drives inhibition of nuclear import, loss of nuclear TDP-43, and cell death. Neuron. 2019, 102(2):339-357. pdf file link cover
J.R. Mann, A.M. Gleixner, J.C. Mauna, E. Gomes, M.R. DeChellis-Marks, P.G. Needham, K.E. Copley, B. Hurtle, B. Portz, N.J. Pyles, L. Guo, C.B. Calder, Z.P. Wills, U.B. Pandey, J.K. Kofler, J.L. Brodsky, A. Thathiah, J. Shorter, and C.J. Donnelly. RNA binding antagonizes neurotoxic phase transitions of TDP-43. Neuron. 2019, 102(2):321-338. pdf file link
Y-J. Zhang, L. Guo, P.K. Gonzales, T.F. Gendron, Y. Wu, K. Jansen-West, A.D. O’Raw, S.R. Pickles, M. Prudencio, Y. Carlomagno, M.A. Gachechiladze, C. Ludwig, R. Tian, J. Chew, M. DeTure, W-L. Lin, J. Tong, L.M. Daughrity, M. Yue, Y. Song, J.W. Andersen, M. Castanedes-Casey, A. Kurti, A. Datta, G. Antognetti, A. McCampbell, R. Rademakers, B. Oskarsson, D.W. Dickson, M. Kampmann, M.E. Ward, J.D. Fryer, C.D. Link, J. Shorter, and L. Petrucelli. Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity. Science. 2019, 363(6428):eaav2606. pdf file link
L. Guo*, C.M. Fare*, and J. Shorter. Therapeutic dissolution of aberrant phases by nuclear-import receptors. Trends. Cell Biol. 2019, 29(4):308-322. pdf file link (*Co-first author).
L. McGurk*, E. Gomes*, L. Guo*, J. Shorter, and N.M. Bonini. Poly(ADP-ribose) engages the TDP-43 nuclear-localization sequence to regulate granulo-filamentous aggregation. Biochemistry. 2018, 57(51):6923−6926. pdf file link (*Co-first author.).
L. McGurk, E. Gomes, L. Guo, J. Mojsilovic-Petrovic, V. Tran, R.G. Kalb, J. Shorter, and N.M. Bonini. Poly(ADP-ribose) prevents aberrant phase separation of TDP-43 by promoting liquid demixing and stress-granule localization. Mol Cell. 2018, 71(5):703-717. pdf file link
E. Bogaert, S. Boeynaems, M. Kato, L. Guo, T.R. Caulfield, J. Steyaert, W. Scheveneels, N. Wilmans, W. Haeck, N. Hersmus, J. Schymkowitz, F. Rousseau, J. Shorter, P. Callaerts, W. Robberecht, P. Van Damme, and L. Van Den Bosch. Molecular dissection of FUS points at synergistic effect of low-complexity domains in toxicity. Cell Rep. 2018, 24(3):529-537 pdf file link cover
L. Guo*, H.J. Kim*, H. Wang*, J. Monaghan°, F. Freyermuth°, J.C. Sung°, K. O’Donovan, C.M. Fare, Z. Diaz, N. Singh, Z.C. Zhang, M. Coughlin, E.A. Sweeny, M.E. DeSantis, M.E. Jackrel, C.B. Rodell, J.A. Burdick, O.D. King, A.D. Gitler, C. Lagier-Tourenne, U.B. Pandey, Y.M. Chook, J.P. Taylor, and J. Shorter. Nuclear-import receptors reverse aberrant phase transitions of RNA-binding proteins with prion-like domains. Cell. 2018, 173(3):677-692. pdf file link (*Co-first author. °Co-second author).
T. Zhang*, Y-C. Wu*, P. Mullane, Y.J. Ji, H. Liu, L. He, A. Arora, H-Y. Hwang, A.F. Alessi, A.G. Niaki, G. Periz, L. Guo, H. Wang, E. Elkayam, L. Joshua-Tor, S. Myong, J.K. Kim, J. Shorter, S-E. Ong, A.K.L. Leung, and J. Wang. FUS regulates activity of microRNA-mediated gene silencing. Mol. Cell. 2018, 69(5):787-801. pdf file link (*Co-first author).
L. Guo and J. Shorter. Biology and pathobiology of TDP-43 and emergent therapeutic strategies. Cold Spring Harb Perspect Med. 2017, 7(9). pii: a024554. pdf file link
L. Guo and J. Shorter. It's raining liquids: RNA tunes viscoelasticity and dynamics of membraneless organelles. Mol. Cell. 2015, 60, 189-192. pdf file link
L. Guo and F. Gai. Simple Method to Enhance the Photostability of the Fluorescence Reporter R6G for Prolonged Single-Molecule Studies. J. Phys. Chem. A. 2013, 117, 6164-6170. pdf link
D. Chow, L. Guo, F. Gai, and M. Goulian. Fluorescence correlation spectroscopy measurements of the membrane protein TetA in Escherichia coli suggest rapid diffusion at short length scales. PLoS ONE. 2012, 7(10):e48600. pdf link
Y-H. Wang, A. Collins, L. Guo, K. B. Smith-Dupont, F. Gai, T. Svitkina and P. A. Janmey. Divalent cation-induced cluster formation by polyphosphoinositides in model membranes. J. Am. Chem. Soc. 2012, 134, 3387-3395. pdf link
L. Guo, K. B. Smith-Dupont and F. Gai. Diffusion as a probe of peptide-induced membrane domain formation. Biochemistry. 2011, 50, 2291-2297. pdf link
J. M. G. Rogers*, A. Polishchuk*, L. Guo*, J. Wang, W. F. DeGrado and F. Gai. Photoinduced electron transfer and fluorophore motion as a probe of the conformational dynamics of membrane proteins: application to the influenza A M2 proton channel. Langmuir. 2011, 27, 3815-3821. (*Equal Contribution). pdf link
L. Guo and F. Gai. Heterogeneous diffusion of a membrane-bound pHLIP peptide. Biophys. J. 2010, 98, 2914-2922. pdf link
K. B. Smith-Dupont*, L. Guo* and F. Gai. Diffusion as a probe of the heterogeneity of antimicrobial peptide-membrane interactions. Biochemistry. 2010, 49, 4672-4678. (*Equal Contribution). pdf link
L. Guo, J. H. Park, T. Lee, P. Chowdhury, M. Lim and F. Gai. Probing the role of hydration in the unfolding transitions of carbonmonoxy myoglobin and apomyoglobin. J. Phys. Chem. B. 2009, 113, 6158-6163. pdf link
S. Mukherjee, M. Waegele, P. Chowdhury, L. Guo and F. Gai. Effect of macromolecular crowding on the folding kinetics of protein secondary structures. J. Mol. Biol. 2009, 393, 227-236. pdf link
L. Guo, P. Chowdhury, J. M. Glasscock and F. Gai. Denaturant induced expansion and compaction of a multi-domain protein: IgG. J. Mol. Biol. 2008, 384, 1029-1036. pdf link
S. Yang, H. Wang, L. Guo, Y. Liu and A. Cao. Interaction of fullerenol with lysozyme investigated by experimental and computational approaches. Nanotechnology. 2008, 19, 395101-395107. pdf link
L. Guo, P. Chowdhury, J. Y. Fang and F. Gai. Heterogeneous and anomalous diffusion inside lipid tubules. J. Phys. Chem. B. 2007, 111, 14244-14249. pdf link
